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targeting vectors pcmv hygro  (Addgene inc)


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    Structured Review

    Addgene inc targeting vectors pcmv hygro
    Targeting Vectors Pcmv Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 678 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/targeting+vectors+pcmv+hygro/hCas9+(Plasmid+%2341815)/bio_rxiv__64898__2026__02__19__706780-345-37-39
    Average 96 stars, based on 678 article reviews
    targeting vectors pcmv hygro - by Bioz Stars, 2026-09
    96/100 stars

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    Knock-Out:

    Article Title: Non-canonical function of the splicing activator U2AF2 in promoting intron retention in the lncRNAs PURPL and MALAT1
    Article Snippet: .. For HCT116 MALAT1 knockout, we used integration by non-homologous end joining, which was accomplished by introducing a simultaneous double-strand break in genomic DNA and in the targeting vectors (pCMV-Puro and pCMV-Hygro)., Plasmids encoding spCas9 and sgRNAs were obtained from Addgene (Plasmids #41815 and #47108). .. Oligonucleotides for construction of sgRNAs were obtained from Integrated DNA Technologies, hybridized, phosphorylated and cloned into the sgRNA plasmid or targeting vector using BbsI sites.

    Non-Homologous End Joining:

    Article Title: Non-canonical function of the splicing activator U2AF2 in promoting intron retention in the lncRNAs PURPL and MALAT1
    Article Snippet: .. For HCT116 MALAT1 knockout, we used integration by non-homologous end joining, which was accomplished by introducing a simultaneous double-strand break in genomic DNA and in the targeting vectors (pCMV-Puro and pCMV-Hygro)., Plasmids encoding spCas9 and sgRNAs were obtained from Addgene (Plasmids #41815 and #47108). .. Oligonucleotides for construction of sgRNAs were obtained from Integrated DNA Technologies, hybridized, phosphorylated and cloned into the sgRNA plasmid or targeting vector using BbsI sites.



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    96
    Addgene inc targeting vectors pcmv hygro
    Targeting Vectors Pcmv Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/targeting+vectors+pcmv+hygro/hCas9+(Plasmid+%2341815)/bio_rxiv__64898__2026__02__19__706780-345-37-39
    Average 96 stars, based on 1 article reviews
    targeting vectors pcmv hygro - by Bioz Stars, 2026-09
    96/100 stars
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